2WVH
Structural insights into the pre-reaction state of pyruvate decarboxylase from Zymomonas mobilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-01 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 70.901, 111.175, 167.118 |
| Unit cell angles | 89.89, 90.87, 101.27 |
Refinement procedure
| Resolution | 16.970 - 2.300 |
| R-factor | 0.172 |
| Rwork | 0.171 |
| R-free | 0.19400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zpd |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.872 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.130 | 0.410 |
| Number of reflections | 798597 | |
| <I/σ(I)> | 11.5 | 4 |
| Completeness [%] | 98.1 | 97.3 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | VAPOUR DIFFUSION. RESERVIOR CONTAINS FLUORIDE 0.2 M AND PEG3350 20% W/V., PH 7.5 |
