2WVG
Structural insights into the pre-reaction state of pyruvate decarboxylase from Zymomonas mobilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 70.277, 92.092, 98.630 |
| Unit cell angles | 73.44, 85.76, 67.82 |
Refinement procedure
| Resolution | 32.040 - 1.750 |
| R-factor | 0.158 |
| Rwork | 0.157 |
| R-free | 0.18700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zpd |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.987 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 1.750 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.120 | 0.470 |
| Number of reflections | 223238 | |
| <I/σ(I)> | 10.7 | 2.3 |
| Completeness [%] | 92.5 | 87.5 |
| Redundancy | 3.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | VAPOUR DIFFUSION. THE RESERVIOR CONTAINING POTASSIUM FLUORIDE 0.2 M AND PEG3350 20% W/V., PH 7.5 |
