2WVC
Structural and mechanistic insights into Helicobacter pylori NikR function
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-02 |
| Detector | ADSC CCD |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 70.848, 70.848, 228.424 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.006 - 2.100 |
| R-factor | 0.2004 |
| Rwork | 0.200 |
| R-free | 0.21450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ca9 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.708 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.780 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.090 | 0.500 |
| Number of reflections | 20773 | |
| <I/σ(I)> | 8.9 | 1.5 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 8.9 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.4-0.7 M AMMONIUM SULFATE, 100 MM CITRATE PH 5-5.4 |
