2WV9
Crystal Structure of the NS3 protease-helicase from Murray Valley encephalitis virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2007-05-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.910, 105.460, 80.068 |
| Unit cell angles | 90.00, 97.42, 90.00 |
Refinement procedure
| Resolution | 43.940 - 2.750 |
| R-factor | 0.268 |
| Rwork | 0.267 |
| R-free | 0.30000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2V8O 2ijo |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.899 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.937 | 2.820 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.090 | 0.730 |
| Number of reflections | 17750 | |
| <I/σ(I)> | 19.2 | 3.2 |
| Completeness [%] | 99.0 | 98 |
| Redundancy | 7.7 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM MES PH 6.0, 20% POLYETHYLENE GLYCOL 6000, 200 MM MAGNESIUM CHLORIDE |
