2WV3

Neuroplastin-55 binds to and signals through the fibroblast growth factor receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X12
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X12
Temperature [K]	288
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	I 2 2 2
Unit cell lengths	49.390, 81.080, 137.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.601 - 1.950
R-factor	0.2097
Rwork	0.208
R-free	0.24860
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.006
RMSD bond angle	1.057
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	HKL2Map
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.600	2.060
High resolution limit [Å]	1.950	1.950
Rmerge	0.060	0.370
Number of reflections	20597
<I/σ(I)>	8.3	2.1
Completeness [%]	99.2	99.6
Redundancy	7.1	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.4		NEUROPLASTIN-55 AT A CONCENTRATION OF 4 MG/ML IN 5 MM SODIUM PHOSPHATE AND 150 MM NACL, PH 7.4, WAS MIXED WITH THE RESERVOIR SOLUTION CONTAINING 13-15% W/V PEG 4000, 0.2 M AMMONIUM SULFATE, AND 0.1 M SODIUM ACETATE, PH 4.6.