2WSW

Crystal Structure of Carnitine Transporter from Proteus mirabilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	H 3
Unit cell lengths	129.198, 129.198, 160.264
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.719 - 2.294
R-factor	0.2105
Rwork	0.209
R-free	0.23440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wit
RMSD bond length	0.007
RMSD bond angle	0.980
Data reduction software	XDS
Data scaling software	SCALA
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.700	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.090
Number of reflections	87500
Completeness [%]	97.9	91.2
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5		pH 5.5