2WSP
Thermotoga maritima alpha-L-fucosynthase, TmD224G, in complex with alpha-L-Fuc-(1-2)-beta-L-Fuc-N3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-04 |
Detector | ADSC CCD |
Spacegroup name | H 3 2 |
Unit cell lengths | 180.249, 180.249, 169.733 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 114.900 - 2.650 |
R-factor | 0.20338 |
Rwork | 0.201 |
R-free | 0.24059 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hl8 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.949 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.790 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.080 | 0.460 |
Number of reflections | 30789 | |
<I/σ(I)> | 16.5 | 3.8 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.7 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 18 % PEG600, 5 % JEFFAMINE M-600, 100 MM TRIS-HCL PH 8.0, 14 MG/ML BETA-L-FUC-N3 PROTEIN CONC. 5 MG/ML |