2WSO
Structure of Cerulean Fluorescent Protein at physiological pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-02 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.990, 62.810, 69.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.570 - 1.150 |
| R-factor | 0.14689 |
| Rwork | 0.145 |
| R-free | 0.17707 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oxe |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.881 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.220 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.040 | 0.450 |
| Number of reflections | 78022 | |
| <I/σ(I)> | 20.4 | 2.8 |
| Completeness [%] | 97.7 | 92.1 |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 15% PEG 8000, 0.1M MGCL2, 0.1 M HEPES PH 7.0 |
