2WSC
Improved Model of Plant Photosystem I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-12 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 120.655, 189.086, 129.388 |
| Unit cell angles | 90.00, 91.24, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.300 |
| R-factor | 0.3627 |
| Rwork | 0.363 |
| R-free | 0.36594 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1YO9 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MR |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.430 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.100 | 0.400 |
| Number of reflections | 88964 | |
| <I/σ(I)> | 17.2 | 2.8 |
| Completeness [%] | 99.0 | 95.2 |
| Redundancy | 4.3 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 22.5 MM MES BIS-TRIS PH 6 10MM SUCCINIC ACID 6% PEG6000 0.015% DTM |
