2WS3
Crystal structure of the E. coli succinate:quinone oxidoreductase (SQR) SdhD Tyr83Phe mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-24 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 119.850, 184.710, 203.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.030 - 3.200 |
| R-factor | 0.22039 |
| Rwork | 0.219 |
| R-free | 0.25271 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wdq |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.301 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.030 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.090 | 0.610 |
| Number of reflections | 71105 | |
| <I/σ(I)> | 12.1 | 2.4 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | PH 8.5 |
