2WS2
The 2 Angstrom structure of a Nu-class GST from Haemonchus contortus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-29 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.216, 101.382, 47.170 |
| Unit cell angles | 90.00, 100.46, 90.00 |
Refinement procedure
| Resolution | 50.710 - 2.010 |
| R-factor | 0.23591 |
| Rwork | 0.233 |
| R-free | 0.28983 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tw9 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.891 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.000 | 2.030 |
| High resolution limit [Å] | 2.010 | 2.000 |
| Rmerge | 0.120 | 0.510 |
| Number of reflections | 28361 | |
| <I/σ(I)> | 124.39 | 2.44 |
| Completeness [%] | 97.7 | 97.2 |
| Redundancy | 4.69 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100MM TRIS HCL, PH 8.5, 50 MM CACL2, 30% (W/V) PEG 10000 |
