2WQJ

Crystal structure of a truncated variant of the human p73 tetramerization domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	57.850, 98.930, 120.120
Unit cell angles	90.00, 91.77, 90.00

Refinement procedure

Resolution	24.960 - 2.000
R-factor	0.2123
Rwork	0.210
R-free	0.24840
Structure solution method	MAD
Starting model (for MR)	NONE
RMSD bond length	0.006
RMSD bond angle	0.892
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.400	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.060	0.370
Number of reflections	90937
<I/σ(I)>	16.2	3.7
Completeness [%]	99.7	99.3
Redundancy	4.8	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	290	SITTING DROP VAPOR DIFFUSION AT 17 DEGREE C. PROTEIN SOLUTION: 10 MG/ML IN 20 MM TRIS (PH 8.5), 50 MM NACL. CRYSTALLIZATION BUFFER: 0.1 M TRIS (PH 8.5), 0.9 M AMMONIUM PHOSPHATE.