2WPW
Tandem GNAT protein from the clavulanic acid biosynthesis pathway (without AcCoA)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.2 |
Synchrotron site | SRS |
Beamline | PX14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-09-09 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.449, 93.501, 126.430 |
Unit cell angles | 90.00, 97.86, 90.00 |
Refinement procedure
Resolution | 32.400 - 2.380 |
R-factor | 0.21151 |
Rwork | 0.209 |
R-free | 0.26563 |
Structure solution method | MAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.019 |
RMSD bond angle | 2.134 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.400 | 2.510 |
High resolution limit [Å] | 2.380 | 2.380 |
Rmerge | 0.130 | 0.350 |
Number of reflections | 68408 | |
<I/σ(I)> | 6.5 | 3 |
Completeness [%] | 92.2 | 71.9 |
Redundancy | 4.6 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 3 MG/ML PROTEIN, 10% PEG8000, 0.1M CACODYLATE PH6, 0.2M SODIUM ACETATE |