2WOV
Trypanosoma brucei trypanothione reductase with bound NADP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-01-13 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.870, 63.450, 169.350 |
| Unit cell angles | 90.00, 97.85, 90.00 |
Refinement procedure
| Resolution | 19.847 - 2.500 |
| R-factor | 0.182 |
| Rwork | 0.179 |
| R-free | 0.25080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2woi |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.730 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.110 | 0.400 |
| Number of reflections | 72546 | |
| <I/σ(I)> | 14.8 | 3.6 |
| Completeness [%] | 93.3 | 85.6 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 15MG/ML PROTEIN IN 25MM HEPES PH 7.5 AND 50MM NABR EQUILIBRATED AGAINST 24% MPD, 10% PEG3350 AND 40MM IMIDAZOLE PH 8.0. |
