2WNR
The structure of Methanothermobacter thermautotrophicus exosome core assembly
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-09-25 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 89.261, 118.233, 154.708 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.860 - 2.650 |
| R-factor | 0.21218 |
| Rwork | 0.211 |
| R-free | 0.25077 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2br2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.257 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.120 | 0.580 |
| Number of reflections | 47654 | |
| <I/σ(I)> | 9.1 | 1.5 |
| Completeness [%] | 98.7 | 92.4 |
| Redundancy | 4.2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 20% PEG3350, 0.1M SUCCINIC ACID PH 7.0, 5% ISOPROPANOL |
