2WNQ
Structure of the E192N mutant of E. coli N-acetylneuraminic acid lyase in space group P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-18 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.630, 142.770, 84.487 |
| Unit cell angles | 90.00, 108.99, 90.00 |
Refinement procedure
| Resolution | 39.950 - 1.800 |
| R-factor | 0.19928 |
| Rwork | 0.197 |
| R-free | 0.25312 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wnn |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.327 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.5.0097) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.590 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.090 | 0.390 |
| Number of reflections | 110985 | |
| <I/σ(I)> | 8.3 | 2.8 |
| Completeness [%] | 98.4 | 97.2 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.2 | 100 MM TRIS-HCL PH 8.2, 200 MM NACL, 18% PEG 3350 |
