2WNB
Crystal Structure of a Mammalian Sialyltransferase in complex with disaccharide and CMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 173 |
Detector technology | IMAGE PLATE |
Collection date | 2008-09-09 |
Detector | MARRESEARCH |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 77.987, 78.347, 99.191 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.340 - 1.550 |
R-factor | 0.17728 |
Rwork | 0.175 |
R-free | 0.21339 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wml |
RMSD bond length | 0.023 |
RMSD bond angle | 2.313 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.610 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.040 | 0.250 |
Number of reflections | 52716 | |
<I/σ(I)> | 19.3 | 3.5 |
Completeness [%] | 85.0 | 83 |
Redundancy | 1.9 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 30% PEG 4000 AND 0.1 M MES, PH 6.8 |