2WLO

POTASSIUM CHANNEL FROM MAGNETOSPIRILLUM MAGNETOTACTICUM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-10
Detector	ADSC CCD
Spacegroup name	I 2 2 2
Unit cell lengths	105.860, 115.360, 289.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.995 - 4.036
R-factor	0.2453
Rwork	0.245
R-free	0.25470
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.016
RMSD bond angle	1.020
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	4.000
Rmerge	0.224
Number of reflections	0
<I/σ(I)>	9.5
Completeness [%]	98.4
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5		PRECIPITANT: 15 % PEG400 2.5 % PEG 4000 2.5 % PEG 8000 10 % GLYCEROL 90 MM HEPES PH 7.5 PROTEIN KIRBAC 3.1, 8MG/ML 150MM KCL 20MM TRIS PH 8.0 20MM TETRABUTYL AMMONIUM BROMIDE 4MM CYMAL-5 0.05% TDM PROTEIN AND PRECIPITANT WERE MIXED TOGETHER IN A 1:1 RATIO, AND EQUILIBRATED AGAINST RESERVOIR SOLUTION IN A SITTING-DROP VAPOUR-DIFFUSION SET-UP.