2WLN
POTASSIUM CHANNEL FROM MAGNETOSPIRILLUM MAGNETOTACTICUM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-31 |
Detector | MARRESEARCH |
Spacegroup name | P 1 |
Unit cell lengths | 106.680, 109.060, 146.390 |
Unit cell angles | 73.95, 82.30, 89.88 |
Refinement procedure
Resolution | 15.000 - 3.440 |
R-factor | 0.2664 |
Rwork | 0.266 |
R-free | 0.27250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xl4 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.671 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.000 | 3.790 |
High resolution limit [Å] | 3.600 | 3.600 |
Rmerge | 0.180 | 0.860 |
Number of reflections | 94851 | |
<I/σ(I)> | 4.1 | 1.6 |
Completeness [%] | 97.0 | 97.1 |
Redundancy | 1.9 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | PRECIPITANT: 15 % PEG 400 2.5 % PEG 4000 2.5 % PEG 8000 10 % GLYCEROL 90 MM HEPES PH 7.5 PROTEIN: KIRBAC3.1, 8MG/ML 150MM KCL 20MM TRIS PH 8.0 0.05% TDM 10MM EDTA 4MM LDAO PROTEIN AND PRECIPITANT WERE MIXED IN A 1:1 RATIO AND EQUILIBRATED AGAINST A RESERVOIR OF PRECIPITANT SOLUTION IN A SITTING DROP VAPOUR DIFFUSION SET-UP. |