2WLG
Crystallographic analysis of the polysialic acid O-acetyltransferase OatWY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 2 2 2 |
| Unit cell lengths | 78.669, 94.393, 100.874 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.900 |
| R-factor | 0.19473 |
| Rwork | 0.193 |
| R-free | 0.23106 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IN-HOUSE STRUCTURE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.342 |
| Data reduction software | HKL |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.050 | 0.280 |
| Number of reflections | 59306 | |
| <I/σ(I)> | 52.8 | 7.2 |
| Completeness [%] | 99.0 | 95.2 |
| Redundancy | 10 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 100 MM SODIUM ACETATE, 2.25 M AMMONIUM ACETATE, pH 4.6 |
