2WLB

Adrenodoxin-like ferredoxin Etp1fd(516-618) of Schizosaccharomyces pombe mitochondria

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU H2B
Temperature [K]	278
Detector technology	IMAGE PLATE
Collection date	2004-04-06
Detector	MAR scanner 345 mm plate
Spacegroup name	P 32 2 1
Unit cell lengths	81.965, 81.965, 68.289
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.184
Rwork	0.182
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1E6E 1ayf 1l6v 2bt6
RMSD bond length	0.012
RMSD bond angle	1.453
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SWISS-MODELLER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.780
High resolution limit [Å]	2.600	2.600
Rmerge	0.110	0.510
Number of reflections	8006
<I/σ(I)>	8.5	3.4
Completeness [%]	99.5	92.4
Redundancy	3.4	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	277	VAPOUR DIFFUSION, SITTING DROP, 4 DEGR. CENTIGRADE. RESERVOIR: 0.04M HEPES,1.8M NA-CITRATE, 3% GLYCEROL,PH7.0. PROTEIN SOLUTION: 18.4MG/ML PROTEIN, 20MM POTASSIUMPHOSPHATE,PH7.4. 0.3 PLUS 0.3 MICROLITER DROPLET.