2WKG
Nostoc punctiforme Debranching Enzyme (NPDE)(Native form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 97 |
| Detector technology | CCD |
| Collection date | 2008-03-16 |
| Detector | ADSC CCD |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 93.731, 93.731, 257.042 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.880 - 3.000 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1joj |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.880 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.080 | 0.310 |
| Number of reflections | 13517 | |
| <I/σ(I)> | 11.3 | 12.8 |
| Completeness [%] | 95.6 | 100 |
| Redundancy | 22.6 | 23.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 20% POLYETHYLENE GLYCOL 8000, 200MM TRIS CL (PH8.5),100MM MGCL2 |
