2WKC

Crystal structure from a single-stranded DNA binding protein from the lactococcal phage p2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-05-10
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	48.280, 71.710, 109.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.028 - 2.600
R-factor	0.197
Rwork	0.192
R-free	0.25090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wkd
RMSD bond length	0.008
RMSD bond angle	1.032
Data scaling software	TRUNCATE
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0088)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.620
High resolution limit [Å]	2.600	2.600
Rmerge	0.110	0.400
Number of reflections	12049
<I/σ(I)>	11.72	3.03
Completeness [%]	98.8	99.8
Redundancy	3.54	3.55

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2		PROTEIN AT 7 MG/ML IN 10 MM TRIS, 300 MM NACL PH 8.0 WAS CRYSTALLIZED BY SITTING-DROP VAPOUR DIFFUSION AGAINST 0.1 M SODIUM CACODYLATE PH 6.2, 45% MME-PEG2000