Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-07 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.160, 85.500, 113.420 |
| Unit cell angles | 96.00, 101.78, 97.46 |
Refinement procedure
| Resolution | 42.000 - 2.300 |
| R-factor | 0.24811 |
| Rwork | 0.246 |
| R-free | 0.29735 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vcv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.172 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.060 | 0.280 |
| Number of reflections | 79974 | |
| <I/σ(I)> | 7.5 | 2.5 |
| Completeness [%] | 92.0 | 65 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | THE CRYSTALS WERE OBTAINED BY HANGING DROP VAPOUR TECHNIQUE BY MIXING 5 UL OF RESERVOIR SOLUTION [100 MM TRIS-HCL PH 7.8, 18% (V/V) PEG 4000, AND 2 MM DITHIOTHREITOL] WITH 5 UL OF PROTEIN SOLUTION (10 MG/ML IN 10 MM TRIS-HCL PH 7.8), 1UL OF 200 MM SPERMINE AND 1 UL OF 25 MM GLUTATHIONE |
