2WJA
Crystal structure of the tyrosine phosphatase Wzb from Escherichia coli K30 in complex with phosphate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 90.030, 90.030, 83.540 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.500 - 2.500 |
| R-factor | 0.2224 |
| Rwork | 0.220 |
| R-free | 0.26840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fek |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.570 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.080 | 0.790 |
| Number of reflections | 12623 | |
| <I/σ(I)> | 27.3 | 5.4 |
| Completeness [%] | 90.8 | 100 |
| Redundancy | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M K2HPO4, 18% W/V PEG 3350, 0.1 M TRIS-CL PH 8.0 |
