2WJ7

human alphaB crystallin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-30
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	49.460, 66.070, 190.970
Unit cell angles	90.00, 92.68, 90.00

Refinement procedure

Resolution	39.034 - 2.631
R-factor	0.2553
Rwork	0.252
R-free	0.32000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wj5
RMSD bond length	0.022
RMSD bond angle	2.071
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	190.000	2.770
High resolution limit [Å]	2.630	2.630
Rmerge	0.220	0.630
Number of reflections	17862
<I/σ(I)>	9.6	1.9
Completeness [%]	99.2	99.2
Redundancy	3.2	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	4 DAYS AT 16 DEGREESC IN 100 MM BICINE PH 9.0, 55% 2-METHYL-2,4-PENTANDIOL, AT A PROTEIN CONCENTRATION OF 20 MG/ML USING 1 UL PROTEIN SOLUTION AND 2 UL RESERVOIR SOLUTION.