2WJ5
Rat alpha crystallin domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-05 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.479, 37.964, 28.081 |
| Unit cell angles | 90.00, 91.55, 90.00 |
Refinement procedure
| Resolution | 28.071 - 1.120 |
| R-factor | 0.1537 |
| Rwork | 0.152 |
| R-free | 0.18020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gme |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.530 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 1.150 |
| High resolution limit [Å] | 1.120 | 1.120 |
| Rmerge | 0.090 | 0.400 |
| Number of reflections | 39225 | |
| <I/σ(I)> | 10.7 | 2.5 |
| Completeness [%] | 91.6 | 51.5 |
| Redundancy | 6.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 289 | SINGLE CRYSTALS OF RAT HSP20 ACD GREW AT 16 DEGREESIN 100 MM MES PH 6.5, BETWEEN 45-52% V/V PEG 200, AT A PROTEIN CONCENTRATION OF AROUND 10 MG/ML USING 1 UL PROTEIN SOLUTION AND 2 UL RESERVOIR SOLUTION. |
