2WIW
Crystal structures of Holliday junction resolvases from Archaeoglobus fulgidus bound to DNA substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-08 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.380, 82.860, 107.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.110 - 1.800 |
| R-factor | 0.1747 |
| Rwork | 0.172 |
| R-free | 0.21970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wcz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.262 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.050 | 0.510 |
| Number of reflections | 27226 | |
| <I/σ(I)> | 15.12 | 2.52 |
| Completeness [%] | 90.1 | 73.9 |
| Redundancy | 3.24 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 35 %(W/V) 1.6-HEXANEDIOL, 0.05 M TRIS.HCL PH=8.5, 0.005 M MAGNESIUM SULFATE |
