2WIQ
Fluorescent protein KillerRed in the native state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-28 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.490, 73.670, 80.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.310 - 2.000 |
| R-factor | 0.17382 |
| Rwork | 0.171 |
| R-free | 0.23564 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gfl |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.882 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.150 | 0.580 |
| Number of reflections | 29194 | |
| <I/σ(I)> | 14.7 | 3.3 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | 1.8M AMMONIUM SULFATE, 0.1M TRIS PH 8.5, 0.05M ASCORBIC ACID, VAPOR DIFFUSION, TEMPERATURE 293K |
