2WIP

STRUCTURE OF CDK2-CYCLIN A COMPLEXED WITH 8-ANILINO-1-METHYL-4,5-DIHYDRO- 1H-PYRAZOLO[4,3-H] QUINAZOLINE-3-CARBOXYLIC ACID

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-D
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2002-04-02
Detector	RIGAKU R-AXIS IV
Spacegroup name	P 62 2 2
Unit cell lengths	184.185, 184.185, 215.005
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.400 - 2.800
R-factor	0.221
Rwork	0.221
R-free	0.25800
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.006
RMSD bond angle	0.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (NULL)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.080	0.300
Number of reflections	50354
<I/σ(I)>	23.8	6.2
Completeness [%]	94.1	95
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				20% AMMONIUM SULPHATE, 1M KCL, 40MM HEPES PH 7