2WIB
Crystal Structures of the N-terminal Intracellular Domain of FeoB from Klebsiella Pneumoniae in GDP binding state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 86.552, 184.688, 38.168 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.530 - 2.560 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | STRUCTURE OF FEOB IN GMPPNP STATE |
| RMSD bond length | 0.034 |
| RMSD bond angle | 3.200 |
| Data scaling software | CrystalClear |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.530 | 2.650 |
| High resolution limit [Å] | 2.560 | 2.560 |
| Rmerge | 0.090 | 0.500 |
| Number of reflections | 19651 | |
| <I/σ(I)> | 23.7 | 3.9 |
| Completeness [%] | 94.5 | 98.2 |
| Redundancy | 6.3 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM TRIS-HCL PH 8.5, 30% PEG8000, 150 MM (NH4)2SO4 |
