2WHN

N-terminal domain from the PilC type IV pilus biogenesis protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Collection date	2009-01-25
Detector	ADSC CCD
Spacegroup name	P 43 21 2
Unit cell lengths	55.168, 55.168, 152.078
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.050
R-factor	0.21899
Rwork	0.216
R-free	0.26994
Structure solution method	MIRAS
Starting model (for MR)	NONE
RMSD bond length	0.004
RMSD bond angle	0.521
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MLPHARE
Refinement software	REFMAC (5.5.0066)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.000	2.160
High resolution limit [Å]	2.050	2.050
Rmerge	0.070	0.440
Number of reflections	14807
<I/σ(I)>	18.7	3.2
Completeness [%]	95.4	86.9
Redundancy	11.5	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1