2WHK
Structure of Bacillus subtilis mannanase man26
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 31 |
| Unit cell lengths | 67.498, 67.498, 72.966 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.480 - 1.700 |
| R-factor | 0.172 |
| Rwork | 0.170 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gw1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.099 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.050 | 0.120 |
| Number of reflections | 40383 | |
| <I/σ(I)> | 44 | 7.1 |
| Completeness [%] | 100.0 | 99 |
| Redundancy | 7 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 35% PEG8K, 0.2M NA ACETATE, 0.1M TRIS PH 7 TO 8 |
