2WH0
Recognition of an intrachain tandem 14-3-3 binding site within protein kinase C epsilon
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-08-13 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.130, 78.160, 108.520 |
| Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
| Resolution | 24.460 - 2.250 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qjb |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.883 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.500 | 2.450 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.100 | 0.410 |
| Number of reflections | 56277 | |
| <I/σ(I)> | 19.4 | 3.9 |
| Completeness [%] | 99.4 | 82.4 |
| Redundancy | 4.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 16 MG/ML PROTEIN 9% (W/V) POLYETHYLENE GLYCOL 3350, 25 MM CALCIUM ACETATE, 25 MM SODIUM FLUORIDE, pH 7.4 |
