2WGY
Crystal structure of the G243A mutant of CYP130 from M. tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-04-04 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.243, 54.093, 43.718 |
| Unit cell angles | 90.00, 96.21, 90.00 |
Refinement procedure
| Resolution | 79.650 - 1.500 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2uuq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.225 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.030 | 0.120 |
| Number of reflections | 59681 | |
| <I/σ(I)> | 45.3 | 9.5 |
| Completeness [%] | 97.0 | 78.2 |
| Redundancy | 3.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.2 | 1.5 M AMMONIUM SULFATE, 0.1 M CITRIC ACID PH 5.2, 3% ISOPROPYL ALCOHOL |
