2WGU
Structure of human adenovirus serotype 37 fibre head in complex with a sialic acid derivative, O-Methyl 5-N- methoxycarbonyl -3,5-dideoxy- D-glycero-a-D-galacto-2-nonulopyranosylonic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-16 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.140, 69.930, 74.450 |
| Unit cell angles | 90.00, 94.35, 90.00 |
Refinement procedure
| Resolution | 74.330 - 1.800 |
| R-factor | 0.1846 |
| Rwork | 0.183 |
| R-free | 0.22348 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uxa |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.339 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.050 | 0.610 |
| Number of reflections | 57656 | |
| <I/σ(I)> | 13.85 | 1.84 |
| Completeness [%] | 99.3 | 98.2 |
| Redundancy | 2.91 | 2.86 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 25-27 % POLYETHYLENE GLYCOL 8000, 50 MM ZINC ACETATE, AND 100 MM HEPES PH7.5 |
