2WGT
Structure of human adenovirus serotype 37 fibre head in complex with a sialic acid derivative, O-Methyl 5-N-propaonyl-3,5-dideoxy-D- glycero-a-D-galacto-2-nonulopyranosylonic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-16 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.270, 69.990, 75.000 |
| Unit cell angles | 90.00, 94.42, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uxa |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.225 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.040 | 0.470 |
| Number of reflections | 58545 | |
| <I/σ(I)> | 18.4 | 2.88 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.74 | 3.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25-27 % POLYETHYLENE GLYCOL 8000, 50 MM ZINC ACETATE, AND 100 MM HEPES PH 7.5 |
