2WGJ

X-ray Structure of PF-02341066 bound to the kinase domain of c-Met

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	95
Detector technology	CCD
Collection date	2006-04-30
Detector	ADSC CCD
Spacegroup name	P 21 21 2
Unit cell lengths	76.926, 94.192, 45.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.960 - 2.000
R-factor	0.21485
Rwork	0.214
R-free	0.23153
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lck
RMSD bond length	0.008
RMSD bond angle	1.081
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	EPMR
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.060	0.290
Number of reflections	18192
<I/σ(I)>	20	2.5
Completeness [%]	78.1	38.5
Redundancy	3.9	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	286	CRYSTALS WERE GROWN BY HANGING DROP VAPOR DIFFUSION AT 13 DEGREES CELCIUS. 1-2 MICROLITERS OF PROTEIN SOLUTION AT 7-15 MG/ML WAS MIXEDWITH AN EQUAL VOLUME OF PRECIPITATING SOLUTION (0-275 MM SODIUM CHLORIDE, 21% (W/V PEG 3350, 50 MM CITRATE-PHOSPHATE PH 4.6)