2WGH
Human Ribonucleotide reductase R1 subunit (RRM1) in complex with dATP and Mg.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-30 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.950, 129.310, 76.670 |
| Unit cell angles | 90.00, 95.50, 90.00 |
Refinement procedure
| Resolution | 48.850 - 2.300 |
| R-factor | 0.1955 |
| Rwork | 0.192 |
| R-free | 0.25762 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zyz |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.613 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.100 | 0.450 |
| Number of reflections | 56722 | |
| <I/σ(I)> | 10.7 | 3 |
| Completeness [%] | 98.4 | 97.7 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
