2WGE
Crystal structure of KasA of Mycobacterium tuberculosis with bound TLM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 77.542, 77.542, 148.458 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.810 - 1.800 |
| R-factor | 0.16902 |
| Rwork | 0.167 |
| R-free | 0.20129 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wgd |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.421 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA ((CCP4)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.060 | 1.900 |
| High resolution limit [Å] | 1.790 | 1.800 |
| Rmerge | 0.060 | 0.320 |
| Number of reflections | 48833 | |
| <I/σ(I)> | 19.1 | 7.5 |
| Completeness [%] | 99.0 | 93.2 |
| Redundancy | 6.7 | 6.45 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2-PROPANOL NACL HEPES PH 7.5 |
