2WGB
Crystal structure of the TetR-like transcriptional regulator LfrR from Mycobacterium smegmatis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 86.646, 86.646, 96.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.323 - 2.000 |
| R-factor | 0.1874 |
| Rwork | 0.185 |
| R-free | 0.22800 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.825 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.400 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.520 |
| Number of reflections | 25569 | |
| <I/σ(I)> | 31.6 | 5.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.5 | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 10% PEG8000, 0.5 M LISO4, pH 8.0 |
