2WG4
Crystal structure of the complex between human hedgehog-interacting protein HIP and sonic hedgehog without calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 88.055, 88.055, 171.946 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.842 - 3.150 |
| R-factor | 0.2364 |
| Rwork | 0.234 |
| R-free | 0.29200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1VHH AND 2WFT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.497 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.250 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.210 | 0.880 |
| Number of reflections | 13878 | |
| <I/σ(I)> | 9.9 | 2.8 |
| Completeness [%] | 99.2 | 99.8 |
| Redundancy | 7.5 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M SODIUM ACETATE, PH 4.6 0.5 M POTASSIUM THIOCYANATE |
