2WFV

Crystal structure of DILP5 variant C4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	40.445, 40.445, 45.241
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.180 - 1.850
R-factor	0.202
Rwork	0.199
R-free	0.23200
Structure solution method	MIR
RMSD bond length	0.013
RMSD bond angle	1.389
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.180	1.900
High resolution limit [Å]	1.850	1.850
Rmerge	0.040	0.440
Number of reflections	3164
<I/σ(I)>	56	4.54
Completeness [%]	100.0	99.6
Redundancy	12.8	10.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				20% PEG4000