2WFR
Crystal structure of the N-terminal signalling domain of human Dhh with calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.130, 41.880, 83.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.880 - 1.950 |
| R-factor | 0.18196 |
| Rwork | 0.180 |
| R-free | 0.22487 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wfq |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.455 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0047) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.050 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.120 | 0.740 |
| Number of reflections | 10765 | |
| <I/σ(I)> | 10.5 | 2.6 |
| Completeness [%] | 93.6 | 91.8 |
| Redundancy | 6.8 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M TRIS-HCL, PH 8.5 0.2 M LITHIUM SULFATE 0.2 M SODIUM THIOCYANATE 30% (W/V) PEG4000 |
