2WFP

Crystal structure of mannose 6-phosphate isomerase (apo form) from Salmonella typhimurium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MAR345
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	35.904, 92.470, 111.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.030 - 1.670
R-factor	0.19
Rwork	0.189
R-free	0.21600
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.009
RMSD bond angle	1.314
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.4.0069)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.660
Rmerge	0.060
Number of reflections	44547
<I/σ(I)>	48.5
Completeness [%]	98.9
Redundancy	19.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		0.1 M MAGNESIUM ACETATE, 0.2 M SODIUM CACODYLATE PH 6.5, 20% PEG 8000 AND 5% DIOXANE