2WFN
Filamin A actin binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 111.500, 125.900, 128.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.160 - 3.200 |
| R-factor | 0.226 |
| Rwork | 0.225 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2eyi |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.014 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.200 | 3.280 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.270 | |
| Number of reflections | 14512 | |
| <I/σ(I)> | 10 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.7 M LITHIUM SULFATE, 0.1 M HEPES PH 7.5 |
