2WFE
Structure of the Candida albicans cytosolic leucyl-tRNA synthetase editing domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 101.060, 43.040, 122.170 |
| Unit cell angles | 90.00, 107.57, 90.00 |
Refinement procedure
| Resolution | 28.830 - 2.900 |
| R-factor | 0.24457 |
| Rwork | 0.241 |
| R-free | 0.30085 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.023 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.020 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.150 | 0.400 |
| Number of reflections | 21666 | |
| <I/σ(I)> | 8.55 | 2.92 |
| Completeness [%] | 94.8 | 71.5 |
| Redundancy | 3 | 1.77 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M AMMONIUM ACETATE, 0.01 M MAGNESIUM ACETATE TETRAHYDRATE, 0.05 M SODIUM CACODYLATE TRIHYDRATE PH 6.5, 30% W/V POLYETHYLENE GLYCOL 8000 |
