2WER
Yeast Hsp90 N-terminal domain LI-IV mutant with Radicicol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 74.007, 74.053, 110.193 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 110.430 - 1.600 |
| R-factor | 0.24471 |
| Rwork | 0.243 |
| R-free | 0.27337 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IN-HOUSE HSP90 N-TERMINAL MODEL. |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.928 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.060 | 0.410 |
| Number of reflections | 80579 | |
| <I/σ(I)> | 15.2 | 2.6 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
