2WEF
Human 3'(2'), 5'-bisphosphate nucleotidase 1 (BPNT1) in complex with AMP, PO4 and Magnesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-01 |
| Detector | ADSC CCD |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 38.980, 67.664, 100.394 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.520 - 1.800 |
| R-factor | 0.146 |
| Rwork | 0.144 |
| R-free | 0.18900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jp4 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.906 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.520 | 1.780 |
| High resolution limit [Å] | 1.800 | 1.690 |
| Rmerge | 0.090 | 0.550 |
| Number of reflections | 30231 | |
| <I/σ(I)> | 20.3 | 3.8 |
| Completeness [%] | 99.5 | 97.2 |
| Redundancy | 13.5 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
