2WE7
Crystal structure of Mycobacterium tuberculosis Rv0376c homologue from Mycobacterium smegmatis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 294 |
| Detector technology | CCD |
| Collection date | 2007-07-11 |
| Detector | ADSC CCD |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 101.039, 101.039, 316.574 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.290 - 2.900 |
| R-factor | 0.198 |
| Rwork | 0.195 |
| R-free | 0.25200 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.288 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.120 | 0.350 |
| Number of reflections | 21926 | |
| <I/σ(I)> | 63.91 | 12.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 26.2 | 23.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M CITRATE PH 4.8, 17% PEG 8000, 0.2 M AMMONIUM ACETATE AND 4% ETHANOL BY MICRO-SEEDING |
